Typically in optical microscopy, the signal-to-noise ratio (SNR) is calculated to quantify the image contrast which indicates how well different regions of interest in the image can be differentiated.

Nearly 100 years ago, a seemingly simple discovery revolutionized the microscope. The introduction of phase contrast, which ...

Light-sheet fluorescence microscopy (LSFM) has allowed for faster three-dimensional imaging of biological samples with lower photo-bleaching and photo-toxicity than confocal microscopy 1. This is ...

Researchers have introduced a solution to the problem of light-sheet fluorescence microscopy: novel illumination beams designed based on deep learning using a trainable phase mask. Their study ...

Fifteen years ago, physicists worked out a method to increase image contrast by hitting the electron imaging beam with an ...

Berkeley Lab and UC Berkeley physicists' new technique offers detailed images of the small molecules and cell structures that ...

(A) When a traditional Gaussian beam is used, diffraction causes the illumination beam to widen at the edges. (B) Joint optimization scheme. A network predicts the positions of beads in the focal ...